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Image Search Results
Journal: iScience
Article Title: Metabolic rewiring and autophagy inhibition correct lysosomal storage disease in mucopolysaccharidosis IIIB
doi: 10.1016/j.isci.2024.108959
Figure Lengend Snippet: Mitochondrial network show signs of disorganization in striatal (upper panels) and cortical (lower panels) regions of NAGLU −/− mice Forty μm-thick coronal brain sections of 8-month-old wild type (WT) and NAGLU −/− mice were stained with the mitochondrial marker mitochondrially encoded cytochrome c oxidase I (MT-CO1, cytochrome c oxidase subunit 1 COX1). Nuclei were stained with Hoechst.
Article Snippet: For Western blot analysis, mouse anti-activated diphosphorylated ERK1/2 monoclonal antibody (M8159) was purchased from Sigma Aldrich Chemical Co., rabbit anti-ERK1/2 polyclonal antibody (V1141) from Promega (USA), mouse anti-phosphorylated Akt monoclonal antibody (sc-514032) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany), mouse anti-Akt polyclonal antibody (sc-5298) Santa Cruz Biotechnology (Heidelberg, Germany), rabbit anti-BCN1 monoclonal antibody (H-300 sc-11427) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany), rabbit anti-LC3 polyclonal antibody (NB100-2331) from Novus Biologicals (USA), mouse anti-GAPDH monoclonal antibody (6C5 sc-32233) from Santa Cruz Biotechnology (Heidelberg, Germany),
Techniques: Staining, Marker
Journal: iScience
Article Title: Metabolic rewiring and autophagy inhibition correct lysosomal storage disease in mucopolysaccharidosis IIIB
doi: 10.1016/j.isci.2024.108959
Figure Lengend Snippet:
Article Snippet: For Western blot analysis, mouse anti-activated diphosphorylated ERK1/2 monoclonal antibody (M8159) was purchased from Sigma Aldrich Chemical Co., rabbit anti-ERK1/2 polyclonal antibody (V1141) from Promega (USA), mouse anti-phosphorylated Akt monoclonal antibody (sc-514032) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany), mouse anti-Akt polyclonal antibody (sc-5298) Santa Cruz Biotechnology (Heidelberg, Germany), rabbit anti-BCN1 monoclonal antibody (H-300 sc-11427) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany), rabbit anti-LC3 polyclonal antibody (NB100-2331) from Novus Biologicals (USA), mouse anti-GAPDH monoclonal antibody (6C5 sc-32233) from Santa Cruz Biotechnology (Heidelberg, Germany),
Techniques: Recombinant, Modification, Protease Inhibitor, Expressing, Clone Assay, Control, Generated, Knock-Out, Software